Proteomic Characterization of Human Neural Stem Cells and Their Secretome During in vitro Differentiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00541278" target="_blank" >RIV/67985904:_____/21:00541278 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/21:00541278 RIV/60461373:22310/21:43923437 RIV/00216208:11310/21:10429936

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fncel.2020.612560/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fncel.2020.612560/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fncel.2020.612560" target="_blank" >10.3389/fncel.2020.612560</a>

Result language

angličtina

Original language name

Proteomic Characterization of Human Neural Stem Cells and Their Secretome During in vitro Differentiation

Original language description

Cell therapies represent a promising approach to slow down the progression of currently untreatable neurodegenerative diseases (e.g., Alzheimer's and Parkinson's disease or amyotrophic lateral sclerosis), as well as to support the reconstruction of functional neural circuits after spinal cord injuries. In such therapies, the grafted cells could either functionally integrate into the damaged tissue, partially replacing dead or damaged cells, modulate inflammatory reaction, reduce tissue damage, or support neuronal survival by secretion of cytokines, growth, and trophic factors. Comprehensive characterization of cells and their proliferative potential, differentiation status, and population purity before transplantation is crucial to preventing safety risks, e.g., a tumorous growth due to the proliferation of undifferentiated stem cells. We characterized changes in the proteome and secretome of human neural stem cells (NSCs) during their spontaneous (EGF/FGF2 withdrawal) differentiation and differentiation with trophic support by BDNF/GDNF supplementation. We used LC-MS/MS in SWATH-MS mode for global cellular proteome profiling and quantified almost three thousand cellular proteins. Our analysis identified substantial protein differences in the early stages of NSC differentiation with more than a third of all the proteins regulated (including known neuronal and NSC multipotency markers) and revealed that the BDNF/GDNF support affected more the later stages of the NSC differentiation. Among the pathways identified as activated during both spontaneous and BDNF/GDNF differentiation were the HIF-1 signaling pathway, Wnt signaling pathway, and VEGF signaling pathway. Our follow-up secretome analysis using Luminex multiplex immunoassay revealed significant changes in the secretion of VEGF and IL-6 during NSC differentiation. Our results further demonstrated an increased expression of neuropilin-1 as well as catenin beta-1, both known to participate in the regulation of VEGF signaling, and showed that VEGF-A isoform 121 (VEGF121), in particular, induces proliferation and supports survival of differentiating cells.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Cellular Neuroscience

ISSN

1662-5102

e-ISSN

1662-5102

Volume of the periodical

14

Issue of the periodical within the volume

JAN 28

Country of publishing house

CH - SWITZERLAND

Number of pages

20

Pages from-to

612560

UT code for WoS article

000616808200001

EID of the result in the Scopus database

2-s2.0-85100712864