The Expression of Selected Wnt Pathway Members (FZD6, AXIN2 and beta-Catenin) in Canine Oral Squamous Cell Carcinoma and Acanthomatous Ameloblastoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00544013" target="_blank" >RIV/67985904:_____/21:00544013 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/21:43879110

Result on the web

<a href="https://www.mdpi.com/2076-2615/11/6/1615" target="_blank" >https://www.mdpi.com/2076-2615/11/6/1615</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani11061615" target="_blank" >10.3390/ani11061615</a>

Result language

angličtina

Original language name

The Expression of Selected Wnt Pathway Members (FZD6, AXIN2 and beta-Catenin) in Canine Oral Squamous Cell Carcinoma and Acanthomatous Ameloblastoma

Original language description

Simple Summary The expression patterns of selected Wnt pathway molecules were analyzed in two of the most common canine oral neoplasia-canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). We found an overlap of areas with high expression of FZD6 and SOX2 in COSCC, while cytokeratin expression was low in these areas, indicating the low differentiation status of these cells. In CAA, FZD6-positive areas expressed cytokeratin and exhibited features of squamous metaplasia. Moreover, the expression of beta-catenin and AXIN2 was higher in both CAA and COSCC than in the healthy canine oral epithelium. This work uncovered the distinct expression patterns of Wnt molecules in both lesions, indicating the involvement of this pathway in the pathology of canine oral cancers, which presents opportunities for their usage for the prediction of cell behavior or in the development of new therapeutic approaches. The Wnt signaling pathway is well known to be involved in many types of human cancer, however, in veterinary medicine, the investigation of individual Wnt members' expression, and their role in or association with oral tumor pathogenesis, is still underevaluated. We aim to determine the expression pattern of Frizzled-6 (FZD6) as one of the Wnt receptors in two of the most common canine oral neoplastic lesions-canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). While COSCC is a malignant tumor with aggressive biological behavior and a tendency to metastasize, CAA is a benign tumor with high local invasiveness. In CAA, the expression of FZD6 was mostly located in the center of the epithelial tumorous tissue, and cells exhibiting features of squamous metaplasia were strongly positive. In well-differentiated COSCC, FZD6 was expressed in the tumorous epithelium as well as the tumorous stroma. There was a negative correlation between cytokeratin expression and FZD6 expression in COSCC, where the central parts of the epithelial tumorous tissue were often FZD6-negative. The non-differentiated COSCC with low expression of cytokeratin exhibited a diffuse FZD6 signal. The invasive front with areas of tumor budding exhibited high FZD6 expression with a loss of cytokeratin expression. Moreover, the expression of beta-catenin and AXIN2 was increased in comparison to gingiva. In conclusion, our study revealed significant differences in the expression patterns and the levels of FZD6 between COSCC and CAA, indicating the differential engagement of the Wnt pathway in these tumors.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

<a href="/en/project/NV19-08-00383" target="_blank" >NV19-08-00383: Prognostic and predictive markers of squamous cell carcinoma invasion in the oral cavity and oropharynx</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animals

ISSN

2076-2615

e-ISSN

2076-2615

Volume of the periodical

11

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

1615

UT code for WoS article

000667025200001

EID of the result in the Scopus database

2-s2.0-85106679623