CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00557508" target="_blank" >RIV/67985904:_____/22:00557508 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/22:00557508 RIV/00216208:11310/22:10455260
Result on the web
<a href="https://journals.biologists.com/jcs/article-abstract/135/6/jcs252924/274776/CDC25B-is-required-for-the-metaphase-I-metaphase?redirectedFrom=fulltext" target="_blank" >https://journals.biologists.com/jcs/article-abstract/135/6/jcs252924/274776/CDC25B-is-required-for-the-metaphase-I-metaphase?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/jcs.252924" target="_blank" >10.1242/jcs.252924</a>

Result language
angličtina
Original language name
CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes
Original language description
Mammalian oocytes are arrested at meiotic prophase I. The dual-specificity phosphatase CDC25B is essential for cyclin-dependent kinase 1 (CDK1) activation that drives resumption of meiosis. CDC25B reverses the inhibitory effect of the protein kinases WEE1 and MYT1 on CDK1 activation. Cdc25b(-/-) female mice are infertile because oocytes cannot activate CDK1. To identify a role for CDC25B following resumption of meiosis, we restored CDK1 activation in Cdc25b(-/-) oocytes by inhibiting WEE1 and MYT1, or expressing EGFP-CDC25A or constitutively active EGFP-CDK1 from microinjected complementary RNAs. Forced CDK1 activation in Cdc25b(-/- )oocytes allowed resumption of meiosis, but oocytes mostly arrested at metaphase I (MI) with intact spindles. Similarly, approximately a third of Cdc25b(-/-)( )oocytes with a reduced amount of CDC25B arrested in MI. MI-arrested Cdc25b(-/-) oocytes also displayed a transient decrease in CDK1 activity similar to Cdc25b(+/-) oocytes during the MI-MII transition, whereas Cdc25b(+/+ )oocytes exhibited only a partial anaphase-promoting complex/cyclosome activation and anaphase I entry. Thus, CDC25B is necessary for the resumption of meiosis and the MI-MII transition.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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