Genome elimination from the germline cells in diploid and triploid male water frogs Pelophylax esculentus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F22%3A00564067" target="_blank" >RIV/67985904:_____/22:00564067 - isvavai.cz</a>
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fcell.2022.1008506/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcell.2022.1008506/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fcell.2022.1008506" target="_blank" >10.3389/fcell.2022.1008506</a>
Alternative languages
Result language
angličtina
Original language name
Genome elimination from the germline cells in diploid and triploid male water frogs Pelophylax esculentus
Original language description
Hybridogenesis is a hemiclonal reproductive strategy in diploid and triploid hybrids. Our study model is a frog P. esculentus (diploid RL and triploids RLL and RRL), a natural hybrid between P. lessonae (LL) and P. ridibundus (RR). Hybridogenesis relies on elimination of one genome (L or R) from gonocytes (G) in tadpole gonads during prespermatogenesis, but not from spermatogonial stem cells (SSCs) in adults. Here we provide the first comprehensive study of testis morphology combined with chromosome composition in the full spectrum of spermatogenic cells. Using genomic in situ hybridization (GISH) and FISH we determined genomes in metaphase plates and interphase nuclei in Gs and SSCs. We traced genomic composition of SSCs, spermatocytes and spermatozoa in individual adult males that were crossed with females of the parental species and gave progeny. Degenerating gonocytes (24%-39%) and SSCs (18%-20%) led to partial sterility of juvenile and adult gonads. We conclude that elimination and endoreplication not properly completed during prespermatogenesis may be halted when gonocytes become dormant in juveniles. After resumption of mitotic divisions by SSCs in adults, these 20% of cells with successful genome elimination and endoreplication continue spermatogenesis, while in about 80% spermatogenesis is deficient. Majority of abnormal cells are eliminated by cell death, however some of them give rise to aneuploid spermatocytes and spermatozoa which shows that hybridogenesis is a wasteful process.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
<a href="/en/project/GA21-25185S" target="_blank" >GA21-25185S: Asexuality as bridge towards polyploidy: evolution of genome and genotype-by-environment interactions under clonality</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cell and Developmental Biology
ISSN
2296-634X
e-ISSN
2296-634X
Volume of the periodical
10
Issue of the periodical within the volume
OCT 14
Country of publishing house
CH - SWITZERLAND
Number of pages
30
Pages from-to
1008506
UT code for WoS article
000878269900001
EID of the result in the Scopus database
2-s2.0-85140909413