Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00640399" target="_blank" >RIV/67985904:_____/25:00640399 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/biolreprod/article-abstract/113/3/605/8157711?redirectedFrom=fulltext&login=true" target="_blank" >https://academic.oup.com/biolreprod/article-abstract/113/3/605/8157711?redirectedFrom=fulltext&login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/biolre/ioaf125" target="_blank" >10.1093/biolre/ioaf125</a>
Alternative languages
Result language
angličtina
Original language name
Programmed DNA elimination occurs during early gametogenesis in hybridogenetic hybrids from Hexagrammos genus
Original language description
Hybridogenesis is a unique type of reproduction found in hybrids, producing offspring that are partial (hemiclonal) genetic replicas of one parent. Along the southern coast of Hokkaido, Japan, two types of interspecies hybrids have been identified among three Hexagrammos species: H. octogrammus (Hoc), H. otakii (Hot), and H. agrammus (Hag). These hybrids are characterized by exclusively fertile females reproducing via hybridogenesis. During oogenesis of hybrids, the paternal haploid genome (Hag or Hot) is excluded, while only the maternal genome (Hoc) retains and transmits to eggs. This study investigates the mechanism of genome elimination in Hexagrammos hybrids through a comparative analysis of gonadal development in 1-year-old juveniles and adult individuals of hybrids and their parental species. Comparative genomic hybridization on whole-mount gonads from Hoc/Hot and (Hoc/Hag) x Hag hybrids revealed gonocytes containing genomes of both parental species and gonocytes with only Hoc genome, suggesting that genome elimination occurs during early gametogenesis. In Hoc but not in Hot or Hag species, we observed heterochromatin foci enriched with H3K9me3 epigenetic histone modification. Similar foci were found in hybrids, confirming the presence of Hoc genome in their gonocytes. Furthermore, we detected micronuclei in the cytoplasm of gonocytes in parental species and hybrids. Although micronuclei were rare, their frequency was significantly higher in hybrids compared to parental species. Within micronuclei in Hoc/Hot and (Hoc/Hag) x Hag hybrids, we identified Hot and Hag chromosomes or their fragments, respectively. Thus, in Hexagrammos hybrids, genome elimination likely occurs during early gametogenic stages and is accompanied by micronuclei formation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
<a href="/en/project/GF23-07028K" target="_blank" >GF23-07028K: Breaking the rules: modification of sexual reproduction pathways in vertebrates.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology of Reproduction
ISSN
0006-3363
e-ISSN
1529-7268
Volume of the periodical
113
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
605-614
UT code for WoS article
001517882800001
EID of the result in the Scopus database
2-s2.0-105016363071