Expression dynamics of metalloproteinases during mandibular bone formation: association with Myb transcription factor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F23%3A00576047" target="_blank" >RIV/67985904:_____/23:00576047 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/23:00131962 RIV/62157124:16170/23:43880625 RIV/00159816:_____/23:00079730

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fcell.2023.1168866/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcell.2023.1168866/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcell.2023.1168866" target="_blank" >10.3389/fcell.2023.1168866</a>

Result language

angličtina

Original language name

Expression dynamics of metalloproteinases during mandibular bone formation: association with Myb transcription factor

Original language description

As the dentition forms and becomes functional, the alveolar bone is remodelled. Metalloproteinases are known to contribute to this process, but new regulators are emerging and their contextualization is challenging. This applies to Myb, a transcription factor recently reported to be involved in bone development and regeneration. The regulatory effect of Myb on Mmps expression has mostly been investigated in tumorigenesis, where Myb impacted the expression of Mmp1, Mmp2, Mmp7, and Mmp9. The aim of this investigation was to evaluate the regulatory influence of the Myb on Mmps gene expression, impacting osteogenesis and mandibular bone formation. For that purpose, knock-out mouse model was used. Gene expression of bone-related Mmps and the key osteoblastic transcription factors Runx2 and Sp7 was analysed in Myb knock-out mice mandibles at the survival limit. Out of the metalloproteinases under study, Mmp13 was significantly downregulated. The impact of Myb on the expression of Mmp13 was confirmed by the overexpression of Myb in calvarial-derived cells causing upregulation of Mmp13. Expression of Mmp13 in the context of other Mmps during mandibular/alveolar bone development was followed in vivo along with Myb, Sp7 and Runx2. The most significant changes were observed in the expression of Mmp9 and Mmp13. These MMPs and MYB were further localized in situ by immunohistochemistry and were identified in pre/osteoblastic cells as well as in pre/osteocytes. In conclusion, these results provide a comprehensive insight into the expression dynamics of bone related Mmps during mandibular/alveolar bone formation and point to Myb as another potential regulator of Mmp13.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Cell and Developmental Biology

ISSN

2296-634X

e-ISSN

2296-634X

Volume of the periodical

11

Issue of the periodical within the volume

Aug 28

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

1168866

UT code for WoS article

001064841000001

EID of the result in the Scopus database

2-s2.0-85170517184