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CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F23%3A00576852" target="_blank" >RIV/67985904:_____/23:00576852 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/23:10474055

  • Result on the web

    <a href="https://www.embopress.org/doi/full/10.15252/embr.202256530" target="_blank" >https://www.embopress.org/doi/full/10.15252/embr.202256530</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.15252/embr.202256530" target="_blank" >10.15252/embr.202256530</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos

  • Original language description

    After fertilization, remodeling of the oocyte and sperm genomes is essential to convert these highly differentiated and transcriptionally quiescent cells into early cleavage-stage blastomeres that are transcriptionally active and totipotent. This developmental transition is accompanied by cell cycle adaptation, such as lengthening or shortening of the gap phases G1 and G2. However, regulation of these cell cycle changes is poorly understood, especially in mammals. Checkpoint kinase 1 (CHK1) is a protein kinase that regulates cell cycle progression in somatic cells. Here, we show that CHK1 regulates cell cycle progression in early mouse embryos by restraining CDK1 kinase activity due to CDC25A phosphatase degradation. CHK1 kinase also ensures the long G2 phase needed for genome activation and reprogramming gene expression in two-cell stage mouse embryos. Finally, Chk1 depletion leads to DNA damage and chromosome segregation errors that result in aneuploidy and infertility.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10605 - Developmental biology

Result continuities

  • Project

    <a href="/en/project/GA20-27742S" target="_blank" >GA20-27742S: Differences in regulation of cell cycle checkpoints between zygote and 2-cell embryo</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Embo Reports

  • ISSN

    1469-221X

  • e-ISSN

    1469-3178

  • Volume of the periodical

    24

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    22

  • Pages from-to

    e56530

  • UT code for WoS article

    001119180600001

  • EID of the result in the Scopus database

    2-s2.0-85170519683