DNA content in Acanthamoeba during two stress defense reactions: Encystation, pseudocyst formation and cell cycle

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903425" target="_blank" >RIV/60076658:12310/21:43903425 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00548210 RIV/00216208:11110/21:10427329
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0932473920300754?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0932473920300754?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ejop.2020.125745" target="_blank" >10.1016/j.ejop.2020.125745</a>

Result language
angličtina
Original language name
DNA content in Acanthamoeba during two stress defense reactions: Encystation, pseudocyst formation and cell cycle
Original language description
During environmental stress, the vegetative cells of the facultative pathogenic amoeba Acanthamoeba castellanii reversibly differentiate into resistant dormant stages, namely, cysts or pseudocysts. The type of resistant stage depends on the nature and duration of the stressor. Cell differentiation is accompanied by changes in morphology and cellular metabolism. Moreover, cell differentiation is also expected to be closely linked to the regulation of the cell cycle and, thus, to cellular DNA content. While the existence of the resistant stages in A. castellanii is well known, there is no consensus regarding the relationship between differentiation and cell cycle progression. In the present work, we used flow cytometry analysis to explore the changes in the DNA content during Acanthamoeba encystation and pseudocyst formation. Our results strongly indicate that A. castellanii enters encystation from the G2 phase of the cell cycle. In contrast, differentiation into pseudocysts can begin in the G1 and G2 phases. In addition, we present a phylogenetic analysis and classification of the main cell cycle regulators, namely, cyclin-dependent kinases and cyclins that are found in the genome of A. castellanii. (C) 2020 Elsevier GmbH. All rights reserved.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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