Mannitol is not involved in protective reactions of Acanthamoeba

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A11363" target="_blank" >RIV/00216208:11110/12:11363 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.molbiopara.2012.04.010" target="_blank" >http://dx.doi.org/10.1016/j.molbiopara.2012.04.010</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mannitol is not involved in protective reactions of Acanthamoeba

Original language description

Genes for mannitol-metabolizing enzymes, mannitol phosphate dehydrogenase (MPDH) and mannitol dehydrogenase (MDH), have been recently identified in the genome of Acanthamoeba castellanii and their potential role in stress tolerance was proposed. Using qRT-PCR, comparison has been made of mRNA levels of the enzymes for mannitol metabolism at various time intervals during the stress defence reactions of encystation and pseudocyst formation. Gradual decrease of both enzymes during encystation and slight increases at the beginning of pseudocyst formation were observed. Detailed analysis of mRNA sequences of the two genes revealed similarities with various alcohol dehydrogenases rather than mannitol dehydrogenases. Our results indicate there is probably noprotective role for mannitol in Acanthamoeba as no mannitol was detected using HILIC ESI MS, in any Acanthamoeba life cycle stage. Possible misinterpretation of previously published sequences as encoding enzymes of the mannitol metabolic

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA310%2F09%2F1120" target="_blank" >GA310/09/1120: Differentiation of free living amoebae of genera Acanthamoeba and Balamuthia</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

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Volume of the periodical

184

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

118-121

UT code for WoS article

000306626300007

EID of the result in the Scopus database

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