Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895643" target="_blank" >RIV/60076658:12310/17:43895643 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/17:00480875 RIV/00216208:11110/17:10366198

Result on the web

<a href="https://ac.els-cdn.com/S1434461017300779/1-s2.0-S1434461017300779-main.pdf?_tid=a5fe162a-f60e-11e7-a4ed-00000aacb361&acdnat=1515593032_79cbffc76bd685116d632a20412f3abc" target="_blank" >https://ac.els-cdn.com/S1434461017300779/1-s2.0-S1434461017300779-main.pdf?_tid=a5fe162a-f60e-11e7-a4ed-00000aacb361&acdnat=1515593032_79cbffc76bd685116d632a20412f3abc</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.protis.2017.09.001" target="_blank" >10.1016/j.protis.2017.09.001</a>

Result language

angličtina

Original language name

Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation

Original language description

The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pseudocyst, formed in reaction to acute stress. The possible role of trehalose in the resistant stages was investigated using a combination of bioinformatic, molecular biological and biochemical approaches. Genes for enzymes from a widespread trehalose-6-synthase-trehalose-6-phosphate phosphatase (TPS-TPP) pathway and a prokaryotic trehalose synthase (TreS) pathway were identified. The expression patterns of the genes during encystation and pseudocyst formation were analyzed and correlated with the time course of cellular trehalose content determined mass spectrometrically. The data clearly demonstrate fundamental differences between encystation and pseudocyst formation at the level of cellular metabolism.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA310%2F09%2F1120" target="_blank" >GA310/09/1120: Differentiation of free living amoebae of genera Acanthamoeba and Balamuthia</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protist

ISSN

1434-4610

e-ISSN

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Volume of the periodical

168

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

649-662

UT code for WoS article

000416347800001

EID of the result in the Scopus database

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