Culture-dependent screening of endospore-forming clostridia in infant feces
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F23%3A00581028" target="_blank" >RIV/67985904:_____/23:00581028 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/23:95000
Result on the web
<a href="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03104-4" target="_blank" >https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03104-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12866-023-03104-4" target="_blank" >10.1186/s12866-023-03104-4</a>
Alternative languages
Result language
angličtina
Original language name
Culture-dependent screening of endospore-forming clostridia in infant feces
Original language description
Background Only a few studies dealt with the occurrence of endospore-forming clostridia in the microbiota of infants without obvious health complications.Methods A methodology pipeline was developed to determine the occurrence of endospore formers in infant feces. Twenty-four fecal samples (FS) were collected from one infant in monthly intervals and were subjected to variable chemical and heat treatment in combination with culture-dependent analysis. Isolates were identified by MALDI-TOF mass spectrometry, 16S rRNA gene sequencing, and characterized with biochemical assays.Results More than 800 isolates were obtained, and a total of 21 Eubacteriales taxa belonging to the Clostridiaceae, Lachnospiraceae, Oscillospiraceae, and Peptostreptococcaceae families were detected. Clostridium perfringens, C. paraputrificum, C. tertium, C. symbiosum, C. butyricum, and C. ramosum were the most frequently identified species compared to the rarely detected Enterocloster bolteae, C. baratii, and C. jeddahense. Furthermore, the methodology enabled the subsequent cultivation of less frequently detectable gut taxa such as Flavonifractor plautii, Intestinibacter bartlettii, Eisenbergiella tayi, and Eubacterium tenue. The isolates showed phenotypic variability regarding enzymatic activity, fermentation profiles, and butyrate production.Conclusions Taken together, this approach suggests and challenges a cultivation-based pipeline that allows the investigation of the population of endospore formers in complex ecosystems such as the human gastrointestinal tract.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Microbiology
ISSN
1471-2180
e-ISSN
1471-2180
Volume of the periodical
23
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
347
UT code for WoS article
001107264700007
EID of the result in the Scopus database
2-s2.0-85176925800