The effects of the coating and aging of biodegradable polylactic acid membranes on in vitro primary human retinal pigment epithelium cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00585554" target="_blank" >RIV/67985904:_____/24:00585554 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/24:00585554 RIV/00216208:11310/24:10487938
Result on the web
<a href="https://www.mdpi.com/2227-9059/12/5/966" target="_blank" >https://www.mdpi.com/2227-9059/12/5/966</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biomedicines12050966" target="_blank" >10.3390/biomedicines12050966</a>
Alternative languages
Result language
angličtina
Original language name
The effects of the coating and aging of biodegradable polylactic acid membranes on in vitro primary human retinal pigment epithelium cells
Original language description
Age-related macular degeneration (AMD) is the most frequent cause of blindness in developed countries. The replacement of dysfunctional human retinal pigment epithelium (hRPE) cells by the transplantation of in vitro-cultivated hRPE cells to the affected area emerges as a feasible strategy for regenerative therapy. Synthetic biomimetic membranes arise as powerful hRPE cell carriers, but as biodegradability is a requirement, it also poses a challenge due to its limited durability. hRPE cells exhibit several characteristics that putatively respond to the type of membrane carrier, and they can be used as biomarkers to evaluate and further optimize such membranes. Here, we analyze the pigmentation, transepithelial resistance, genome integrity, and maturation markers of hRPE cells plated on commercial polycarbonate (PC) versus in-house electrospun polylactide-based (PLA) membranes, both enabling separate apical/basolateral compartments. Our results show that PLA is superior to PC-based membranes for the cultivation of hRPEs, and the BEST1/RPE65 maturation markers emerge as the best biomarkers for addressing the quality of hRPE cultivated in vitro. The stability of the cultures was observed to be affected by PLA aging, which is an effect that could be partially palliated by the coating of the PLA membranes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedicines
ISSN
2227-9059
e-ISSN
2227-9059
Volume of the periodical
12
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
966
UT code for WoS article
001232415400001
EID of the result in the Scopus database
2-s2.0-85194074481