Loss of ADAR1 protein induces changes in small RNA landscape in hepatocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F24%3A00597898" target="_blank" >RIV/67985904:_____/24:00597898 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10481358 RIV/00216208:11410/24:10481358
Result on the web
<a href="https://rnajournal.cshlp.org/content/30/9/1164" target="_blank" >https://rnajournal.cshlp.org/content/30/9/1164</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.080097.124" target="_blank" >10.1261/rna.080097.124</a>
Alternative languages
Result language
angličtina
Original language name
Loss of ADAR1 protein induces changes in small RNA landscape in hepatocytes
Original language description
In recent years, numerous evidence has been accumulated about the extent of A-to-I editing in human RNAs and the key role ADAR1 plays in the cellular editing machinery. It has been shown that A-to-I editing occurrence and frequency are tissue-specific and essential for some tissue development, such as the liver. To study the effect of ADAR1 function in hepatocytes, we have created Huh7.5 ADAR1 KO cell lines. Upon IFN treatment, the Huh7.5 ADAR1 KO cells show rapid arrest of growth and translation, from which they do not recover. We analyzed translatome changes by using a method based on sequencing of separate polysome profile RNA fractions. We found significant changes in the transcriptome and translatome of the Huh7.5 ADAR1 KO cells. The most prominent changes include negatively affected transcription by RNA polymerase III and the deregulation of snoRNA and Y RNA levels. Furthermore, we observed that ADAR1 KO polysomes are enriched in mRNAs coding for proteins pivotal in a wide range of biological processes such as RNA localization and RNA processing, whereas the unbound fraction is enriched mainly in mRNAs coding for ribosomal proteins and translational factors. This indicates that ADAR1 plays a more relevant role in small RNA metabolism and ribosome biogenesis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RNA
ISSN
1355-8382
e-ISSN
1469-9001
Volume of the periodical
30
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
1164-1183
UT code for WoS article
001293563700001
EID of the result in the Scopus database
2-s2.0-85201727703