Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00493723" target="_blank" >RIV/68081715:_____/18:00493723 - isvavai.cz</a>
Result on the web
<a href="https://www.jove.com/video/58079" target="_blank" >https://www.jove.com/video/58079</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/58079" target="_blank" >10.3791/58079</a>

Result language
angličtina
Original language name
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Original language description
Studies in dynamic changes in protein translation require specialized methods. In this paper, we examine changes in newly-synthesized proteins in response to ischemia and reperfusion using the isolated perfused mouse heart coupled with polysome profiling. We present the methods that were used to analyze the distribution of proteins, mRNAs, and miRNAs in the fractions after sucrose gradient centrifugation. Specifically, in the high-efficiency fractions (numerous ribosomes attached to mRNA), low-efficiency fractions (fewer ribosomes attached), and the non-translating fractions.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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