Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00639475" target="_blank" >RIV/67985904:_____/25:00639475 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00142388 RIV/62157124:16270/25:43882467 RIV/00027162:_____/25:N0000039
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0889157525006702?pes=vor&utm_source=clarivate&getft_integrator=clarivate" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0889157525006702?pes=vor&utm_source=clarivate&getft_integrator=clarivate</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2025.107855" target="_blank" >10.1016/j.jfca.2025.107855</a>
Alternative languages
Result language
angličtina
Original language name
Detection and quantification of the canine DNA in meat DNA mixes and meat mixtures by real time PCR and digital droplet PCR
Original language description
Accurate detection of animal species in meat products is essential for food safety, regulatory compliance, and the prevention of food fraud. This study presents the development and validation of a modified quadruplex quantitative PCR (qPCR) assay and singleplex droplet digital PCR (ddPCR) for the detection and quantification of canine DNA in meat and DNA mixtures. Two- and three-species samples containing canine, pork, and chicken components (0.1 %-100 %) were prepared, including heat-treated variants. A simplified quantification approach using a single 100 % DNA reference diluted to 10 ng/mu L was applied in both methods. Results from qPCR and ddPCR were statistically evaluated using ANOVA, RMSD, and coefficient of variation (CV). Both methods yielded accurate results across most concentration ranges, with no significant differences in calculated percentages. However, qPCR exhibited significantly higher variability than ddPCR, especially at extreme concentrations (<1 % and >99 %). ddPCR showed superior performance in three-species mixtures and yielded lower RMSD values overall. Despite higher variability at detection limits inherent to PCR technology, both methods are suitable for routine quantification of canine DNA in meat products. The study confirms that a single-reference standard is a cost-effective and reliable alternative to full calibration curves for species quantification.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food Composition and Analysis
ISSN
0889-1575
e-ISSN
1096-0481
Volume of the periodical
146
Issue of the periodical within the volume
OCT 25
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
107855
UT code for WoS article
001568377000003
EID of the result in the Scopus database
2-s2.0-105007061570