Subretinal Injection Volume Correlates to Persistent Outer Retinal Thinning in the Pig Eye
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00642524" target="_blank" >RIV/67985904:_____/25:00642524 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10505854
Result on the web
<a href="https://iovs.arvojournals.org/article.aspx?articleid=2811074" target="_blank" >https://iovs.arvojournals.org/article.aspx?articleid=2811074</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1167/iovs.66.14.27" target="_blank" >10.1167/iovs.66.14.27</a>
Alternative languages
Result language
angličtina
Original language name
Subretinal Injection Volume Correlates to Persistent Outer Retinal Thinning in the Pig Eye
Original language description
PURPOSE. Transgenic pig models are a valuable model for preclinical testing of gene and cell therapies. Subretinal injection (SRI) is a common drug delivery method but has been associated with retinal thinning and atrophy. This study examined whether SRI volume correlates with retinal thinning in the pig eye and compared the effects of balanced salt solution (BSS) and adeno-associated virus (AAV) injections. METHODS. Ten eyes from five transgenic pigs were included in this study. Eight eyes received escalating subretinal injection volumes (SRVs) (50, 100, 150, and 200 mu L) of either BSS or AAV [5 x 10(11 )vg/mL], targeting the cone-rich area centralis of the pig eye. After six months, outer retinal thickness inside versus outside the bleb area (AORT) was quantified using optical coherence tomography (OCT). Histology was performed to confirm OCT findings. RESULTS. Treated eyes showed clinically relevant (-21.5 +/- 2.7 mu m) outer retinal thinning inside the bleb area (P = 0.0001). A strong, statistically significant, linear correlation (R-2 = 0.73 P = 0.0068) was found between SRV and ORT loss. There was a 1 mu m loss of ORT for every 9 mu L of SRV. ORT loss was similar between AAV and BSS, except at the highest volume (200 mu L, 1 x 10(11) vg), where greater thinning occurred with AAV over BSS (A11 mu m). CONCLUSIONS. This study supports the notion that SRV could be an independent factor in development of outer retinal thinning in the pig eye. Modifying surgical technique to favor the placement of multiple smaller blebs of <100 mu L might mitigate retinal thinning because of volumetric stress and enhance the preclinical safety profile of investigational therapies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
<a href="/en/project/EH22_008%2F0004562" target="_blank" >EH22_008/0004562: Excellent Research in Regenerative Medicine</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Investigative Ophthalmology and Visual Science
ISSN
0146-0404
e-ISSN
1552-5783
Volume of the periodical
66
Issue of the periodical within the volume
14
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
27
UT code for WoS article
001622822500005
EID of the result in the Scopus database
2-s2.0-105021544885