Mitochondria as indispensable yet replaceable components of germ plasm: insights into PGCs specification in sturgeons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00643129" target="_blank" >RIV/67985904:_____/25:00643129 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/25:00643129 RIV/60076658:12520/25:43909683

Result on the web

<a href="https://rep.bioscientifica.com/view/journals/rep/169/4/REP-24-0441.xml" target="_blank" >https://rep.bioscientifica.com/view/journals/rep/169/4/REP-24-0441.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-24-0441" target="_blank" >10.1530/REP-24-0441</a>

Result language

angličtina

Original language name

Mitochondria as indispensable yet replaceable components of germ plasm: insights into PGCs specification in sturgeons

Original language description

In brief The mitochondria within germ plasm contribute to the formation and specification of primordial germ cells (PGCs) in non-teleost fishes regardless of their origin from germ plasm. This study offers new insights into germ cell biology and potential strategies for conserving matrilineal genetics in sturgeons.Abstract While it is widely recognised that mitochondria are components of germ plasm, their specific role in the formation and specification of PGCs remains poorly understood. Furthermore, it has not been established whether mitochondria in germ plasm possess unique characteristics essential for their function. In this study, we demonstrate that mitochondria are indispensable for PGC development in non-teleost fishes and that their role is not dependent on their origin from germ plasm. Using sturgeon embryos, we showed that UV radiation applied to the vegetal pole effectively eliminates germ plasm, including mitochondria, and prevents PGC formation. Remarkably, we restored germ plasm function and PGC development by injecting mitochondria derived from donor eggs, even when these mitochondria were not originally part of the germ plasm. Transplanted mitochondria were successfully identified in larval PGCs using a fluorescent PKH26 tracer, and in interspecies transplantation experiments, their presence was confirmed using species-specific mtDNA and mtRNA primers in larvae and individual PGCs. Our findings reveal that mitochondria are critical but not germ plasm-specific determinants of PGC formation. This study provides novel insights into the developmental pathways of germ cells and establishes a previously unrecognised flexibility in mitochondrial functionality within the germ line. These findings also offer a potential method for conserving matrilineal genetics in critically endangered species such as sturgeons while simultaneously opening new avenues for studying germ lines with high interspecies mitochondrial heteroplasmy and contributing to broader evolutionary and conservation biology.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproduction

ISSN

1470-1626

e-ISSN

1741-7899

Volume of the periodical

169

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

e240441

UT code for WoS article

001518870400014

EID of the result in the Scopus database

2-s2.0-105001219425