Beyond the Net: Optimizing eDNA Sampling and Analysis for Challenging Muddy Habitats and the Endangered Weatherfish (Misgurnus fossilis)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00645424" target="_blank" >RIV/67985904:_____/25:00645424 - isvavai.cz</a>
Result on the web
<a href="https://aca.pensoft.net/article/180307/" target="_blank" >https://aca.pensoft.net/article/180307/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3897/aca.8.e180307" target="_blank" >10.3897/aca.8.e180307</a>
Alternative languages
Result language
angličtina
Original language name
Beyond the Net: Optimizing eDNA Sampling and Analysis for Challenging Muddy Habitats and the Endangered Weatherfish (Misgurnus fossilis)
Original language description
Elusive and endangered species are prime candidates for environmental DNA (eDNA) monitoring (Duarte et al. 2023). The European weatherfish (Misgurnus fossilis) is one such species where traditional fishing methods may not yield conclusive results on its presence/absence. On the other hand, eDNA monitoring is less labour-intensive than conventional methods and may require only a small amount of water if species-specific qPCR/ddPCR markers are utilised (Thomsen et al. 2011, Sigsgaard et al. 2015, Kusanke et al. 2020, Brys et al. 2020). We aim to develop a standardised eDNA protocol that would provide additional information to traditional fishing surveys of Misgurnus . Such surveys are especially challenging in smaller vegetated waterbodies and wetlands in Central Europe where electrofishing, dip-netting, or fish trapping may not be feasible. As such, these wetland habitats are also among the most neglected European habitats despite facing considerable threats (Šmejkal et al. 2025).
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
<a href="/en/project/SS07010366" target="_blank" >SS07010366: Genetic diversity of the weather loach (Misgurnus fossilis) in the Czech Republic – providing fundamental data for conservation measures of a disappearing, critically endangered species</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
ARPHA Conference Abstracts
ISBN
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ISSN
2603-3925
e-ISSN
2603-3925
Number of pages
2
Pages from-to
e180307
Publisher name
ARPHA
Place of publication
-
Event location
Vídeň
Event date
Oct 23, 2025
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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