Metabolic activity and membrane integrity changes in Microcystis aeruginosa ? new findings on hydrogen peroxide toxicity in cyanobacteria

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F12%3A00379236" target="_blank" >RIV/67985939:_____/12:00379236 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/09670262.2012.687144" target="_blank" >http://dx.doi.org/10.1080/09670262.2012.687144</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09670262.2012.687144" target="_blank" >10.1080/09670262.2012.687144</a>

Result language
angličtina
Original language name
Metabolic activity and membrane integrity changes in Microcystis aeruginosa ? new findings on hydrogen peroxide toxicity in cyanobacteria
Original language description
The aim of our study was to investigate the intracellular toxicity mechanisms of potentially anti-cyanobacterial agent hydrogen peroxide in Microcystis aeruginosa. Metabolic activity and cell membrane integrity were evaluated by flow cytometry in cyanobacteria exposed to the substance tested in the dark or light; the relationships between exposure effects and the kinetics of hydrogen peroxide decomposition were studied. Flow cytometry and chlorophyll a fluorescence measurements suggested that hydrogen peroxide exposure elicits an immediate decline of metabolic activity, and immediate changes of chlorophyll a fluorescence parameters, followed later by an increase in the percentage of membrane-compromised cells. When the concentration of hydrogen peroxide used was lethal, a significant drop in total cell counts was detected. Our study also confirmed that light is the critical factor affecting peroxide decomposition and thus greatly influences its toxicity.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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