HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00051723" target="_blank" >RIV/00216224:14110/10:00051723 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS
Original language description
The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic ?DNA comets?assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant ? N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FP - Other medical fields
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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