Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00024908" target="_blank" >RIV/61389030:_____/05:00024908 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis
Original language description
We applied the nuclear DNA Diffusion Assay, described as an accurate tool to estimate apoptotic and necrotic cells [N.P. Singh, A simple method for accurate estimation of apoptotic cells, Exp. Cell Res. 256 (2000) 328-337] to tobacco root and leaf cells.In this assay, isolated nuclei are embedded in an agarose microgel on a microscope slide and low molecular-weight DNA fragments diffuse into the microgel. Exposure of the roots to hydrogen peroxide significantly increased the average nuclear area of isolated nuclei. After 4 and 24h of recovery, all DNA damage was repaired. The data clearly demonstrate that the manifestation of diffused nuclei upon exposure to hydrogen peroxide is not the result of non-repairable apo-ptotic or necrotic DNA fragmentation, but represents repairable genotoxin-induced DNA da-mage. In contrast, treatment with the alkylating agent ethyl methanesulphonate (EMS) fol-lowed by 24 h of recovery produced a significant increase in the average nuclear area. The co...
Czech name
Hodnocení jaderného DNA difúzního testu k detekci apoptózy a apoptózy
Czech description
Difuzní jaderní test je doporučen k rozlišení apoptotického a nekrotického poškození DNA podle struktury jader u savčích buněk. Tento test jsme aplikovali na jádra z listů a kořenů tabáku (Nicotiana tabacum var. xanthi). Po aplikaci peroxidu vodíku na jádra z kořenů se průkazně zvýšil průměr jader, způsobený poškozením DNA, po 24 h však bylo poškození DNA kompletně napraveno. To dokazuje, že poškození DNA není podmíněno apoptózou nebo apoptózou , která není reparovatelná, ale reparovatelnou genotoxicitou. Ani po aplikaci DNase-1 a hyperthermie nebylo možno rozlišit podle struktury mezi apoptotickou, nekrotickou a genotoxickou fragmentaci DNA.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA521%2F05%2F0500" target="_blank" >GA521/05/0500: Induced DNA damage, mutagenicity, homologous recombination and apoptosis as endpoints for monitoring effects of pollutants in tobacco and potato</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
ISSN
1383-5718
e-ISSN
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Volume of the periodical
586
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
38-46
UT code for WoS article
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EID of the result in the Scopus database
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