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A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F13%3A00393243" target="_blank" >RIV/67985939:_____/13:00393243 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1371/journal.pone.0055283" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0055283</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1371/journal.pone.0055283" target="_blank" >10.1371/journal.pone.0055283</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes

  • Original language description

    Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EH - Ecology - communities

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PLoS ONE

  • ISSN

    1932-6203

  • e-ISSN

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

  • UT code for WoS article

    000315184200014

  • EID of the result in the Scopus database