The application of RNA-seq to the comprehensive analysis of plant mitochondrial transcriptomes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F15%3A00451029" target="_blank" >RIV/67985939:_____/15:00451029 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/15:00446185

Result on the web

<a href="http://dx.doi.org/10.1007/s00438-014-0905-6" target="_blank" >http://dx.doi.org/10.1007/s00438-014-0905-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00438-014-0905-6" target="_blank" >10.1007/s00438-014-0905-6</a>

Result language

angličtina

Original language name

The application of RNA-seq to the comprehensive analysis of plant mitochondrial transcriptomes

Original language description

We review current studies of plant mitochondrial transcriptomes performed by RNA-seq, highlighting methodological challenges unique to plant mitochondria. We propose ways to improve read mapping accuracy and sensitivity such as modifying a reference genome at RNA editing sites, using splicing- and ambiguity-competent aligners, and masking chloroplast- or nucleus-derived sequences. We also outline modified RNA-seq methods permitting more accurate detection and quantification of partially edited sites andthe identification of transcription start sites on a genome-wide scale. The application of RNA-seq goes beyond genome-wide determination of transcript levels and RNA maturation events, and emerges as an elegant resource for the comprehensive identification of editing, splicing, and transcription start sites. Thus, improved RNA-seq methods customized for plant mitochondria hold tremendous potential for advancing our understanding of plant mitochondrial evolution and cyto-nuclear interact

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

<a href="/en/project/GAP506%2F12%2F1359" target="_blank" >GAP506/12/1359: The control of flowering in Chenopodium investigated by the transcriptomic approach</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Genetics and Genomics

ISSN

1617-4615

e-ISSN

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Volume of the periodical

290

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1-9

UT code for WoS article

000348923900001

EID of the result in the Scopus database

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