Development of SSR Markers by 454 Sequencing in the Endemic Species Gentianella praecox subsp. bohemica (Gentianaceae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F17%3A00477571" target="_blank" >RIV/67985939:_____/17:00477571 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/17:10368581
Result on the web
<a href="http://dx.doi.org/10.3732/apps.1600114" target="_blank" >http://dx.doi.org/10.3732/apps.1600114</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3732/apps.1600114" target="_blank" >10.3732/apps.1600114</a>
Alternative languages
Result language
angličtina
Original language name
Development of SSR Markers by 454 Sequencing in the Endemic Species Gentianella praecox subsp. bohemica (Gentianaceae)
Original language description
Gentianella praecox subsp. bohemica (Gentianaceae) is a highly protected taxon in Europe. Polymorphic microsatellite loci were developed and used to genotype individuals of Gentianella praecox subsp. bohemica (Gentianaceae) to study the genetic structure of the remaining populations. Thirty-eight primer pairs were successfully amplified. Of these, 12 polymorphic microsatellite loci were developed using a 454 sequencing approach and used to genotype 180 individuals of G. praecox subsp. bohemica from six populations. Allelic richness ranged between one and nine alleles per locus. We detected a high frequency of polyploid individuals (77.8%). The highest average percentage of heterozygous genotypes was identified for samples from the Hroby population (75.5%). All loci can also be amplified in the congeneric species G. praecox subsp. praecox, G. amarella subsp. amarella, and G. obtusifolia subsp. sturmiana.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applications in Plant Sciences
ISSN
2168-0450
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
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UT code for WoS article
000393800800006
EID of the result in the Scopus database
2-s2.0-85009063611