Development of microsatellite markers for the perennial plant Tofieldia calyculata
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F20%3A00533590" target="_blank" >RIV/67985939:_____/20:00533590 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10422579
Result on the web
<a href="https://doi.org/10.1002/aps3.11327" target="_blank" >https://doi.org/10.1002/aps3.11327</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/aps3.11327" target="_blank" >10.1002/aps3.11327</a>
Alternative languages
Result language
angličtina
Original language name
Development of microsatellite markers for the perennial plant Tofieldia calyculata
Original language description
Polymorphic microsatellite markers were developed to study genetic diversity and genetic structure of populations of the locally endangered species Tofieldia calyculata (Tofieldiaceae). Nineteen polymorphic microsatellite loci were developed using DNA-enriched libraries sequenced by Ilumina technology and were then used to genotype 101 individuals from five populations from Austria, Slovakia, Poland, and the Czech Republic. Of the markers tested, 68% were polymorphic in four of the five investigated populations, with one marker polymorphic in all populations. The number of alleles per locus in the populations ranged from one to 11. Levels of observed and expected heterozygosity ranged from 0.00 to 0.75 and from 0.00 to 0.84, respectively. Moreover, six of the 19 loci amplified when tested in the congeneric species T. pusilla. The 19 newly developed microsatellite markers can be used to describe genetic diversity and population structure of populations of T. calyculata.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applications in Plant Sciences
ISSN
2168-0450
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
1-5
UT code for WoS article
000520981900001
EID of the result in the Scopus database
2-s2.0-85081742830