Metabarcoding of soil environmental DNA to estimate plant diversity globally
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F23%3A00572140" target="_blank" >RIV/67985939:_____/23:00572140 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/23:43906477
Result on the web
<a href="https://doi.org/10.3389/fpls.2023.1106617" target="_blank" >https://doi.org/10.3389/fpls.2023.1106617</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2023.1106617" target="_blank" >10.3389/fpls.2023.1106617</a>
Alternative languages
Result language
angličtina
Original language name
Metabarcoding of soil environmental DNA to estimate plant diversity globally
Original language description
Traditional approaches to collecting large-scale biodiversity data pose huge logistical and technical challenges. We assessed how a comparatively simple method based on sequencing a short fragment (P6 loop) of the chloroplast trnL intron from soil environmental DNA (eDNA) characterises global variation in plant diversity and community composition compared with data derived from traditional plant inventory methods. Large-scale plant diversity and community composition patterns revealed by sequencing eDNA from 325 globally distributed soil samples were broadly in accordance with those derived from sources based on empirical (GBIF) or extrapolated plant distribution and diversity data. The success of the eDNA taxonomy assignment, and the overlap of taxon lists between eDNA and GBIF, was greatest at moderate to high latitudes of the northern hemisphere. On average, around half (mean: 51.5% SD 17.6) of local GBIF records were represented in eDNA databases at the species level, depending on the geographic region. Important experimental considerations for plant eDNA studies include using a sampling volume and design to maximise the number of taxa detected and optimising the sequencing depth. However, increasing the coverage of reference sequence databases would yield the most significant improvements in the accuracy of taxonomic assignments made using the P6 loop of the trnL region
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
1664-462X
Volume of the periodical
14
Issue of the periodical within the volume
APR 18 2023
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
1106617
UT code for WoS article
000980078800001
EID of the result in the Scopus database
2-s2.0-85159908348