A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F24%3A00597609" target="_blank" >RIV/67985939:_____/24:00597609 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00136658
Result on the web
<a href="https://doi.org/10.1093/jat/bkad092" target="_blank" >https://doi.org/10.1093/jat/bkad092</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jat/bkad092" target="_blank" >10.1093/jat/bkad092</a>
Alternative languages
Result language
angličtina
Original language name
A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples
Original language description
Saxitoxins (STXs) are potent neurotoxins produced by marine dinoflagellates or freshwater cyanobacteria, known to cause acute and eventually fatal human intoxications, which are classified as paralytic shellfish poisonings (PSPs). Rapid analysis of STXs in blood plasma can be used for a timely diagnosis and confirmation of PSPs. We developed a fast and simple method of STX extraction based on plasma sample acidification and precipitation by acetonitrile, followed by quantification using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Our approach provides the results <= 30 min, with a limit of detection of 2.8 ng/mL and a lower limit of quantification of 5.0 ng/mL. Within-run and between-run precision experiments showed good reproducibility with <= 15% values. Standard curves for calibration were linear with correlation coefficients >= 0.98 across the assay calibration range (5-200 ng/mL). In an interlaboratory analytical exercise, the method was found to be 100% accurate in determining the presence or absence of STX in human plasma specimens, with recovery values of 86-99%. This simple method for STX determination in animal or human plasma can quickly and reliably diagnose STX exposures and confirm suspected PSP cases to facilitate patient treatment or expedite necessary public health or security actions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Analytical Toxicology
ISSN
0146-4760
e-ISSN
1945-2403
Volume of the periodical
48
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
119-125
UT code for WoS article
001146868300001
EID of the result in the Scopus database
2-s2.0-85186540329