Simple validated method of LC-MS/MS determination of BZ agent in rat plasma samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555475" target="_blank" >RIV/60162694:G44__/20:00555475 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/20:10408006
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/dta.2742" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/dta.2742</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/dta.2742" target="_blank" >10.1002/dta.2742</a>
Alternative languages
Result language
angličtina
Original language name
Simple validated method of LC-MS/MS determination of BZ agent in rat plasma samples
Original language description
Agent BZ (3-quinuclidinyl benzilate) is a centrally acting synthetic anticholinergic agent, considered as a potential military incapacitating chemical warfare agent. Despite its significance as a model compound in pharmacological research and its potential misuse in chemical attacks, few modern analytical methods for BZ determination in biological samples have been published. The goal of the present work is to develop and validate a sensitive and rapid LC-MS/MS method for the determination of agent BZ in rat plasma. The sample preparation was based on solid-phase extraction on C-18 cartridges. The reversed-phase HPLC coupled with the mass spectrometer with electrospray ionization in the positive ion-selective reaction monitoring mode was employed in the BZ analysis. Atropine was used as an internal standard. The presented method is selective, accurate, precise, and linear (r(2) = 0.9947) in a concentration range from 0.5 ng/mL to 1 000 ng/mL and sensitive enough (limit of detection 0.2 ng/mL; limit of quantification 0.5 ng/mL) to determine the BZ plasma levels in rats exposed to 2 mg/kg and 10 mg/kg of BZ. The highest level of BZ in plasma was observed 5 minutes after intramuscular administration (154.6 +/- 22.3 ng/mL in rats exposed to 2 mg/kg of BZ and 1024 +/- 269 ng/mL in rats exposed to 10 mg/kg). After 48 h, no BZ was observed at detectable levels. This new method allows the detection and quantification of BZ in biological samples after exposure of an observed organism and it will be further optimized for other tissues to observe the distribution of BZ in organs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Drug Testing and Analysis
ISSN
1942-7603
e-ISSN
1942-7611
Volume of the periodical
12
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
431-438
UT code for WoS article
000506523800001
EID of the result in the Scopus database
2-s2.0-85077854014