DNA and PNA sensing on mercury and carbon electrodes by using methylene blue as an electrochemical label.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F02%3A17023034" target="_blank" >RIV/68081707:_____/02:17023034 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DNA and PNA sensing on mercury and carbon electrodes by using methylene blue as an electrochemical label.

Original language description

The study is dealing with methylene blue (MB) binding to DNA at hanging mercury drop electrode (HMDE), glassy carbon electrode (GCE), and carbon paste electrode (CPE) in the solution and at the electrode surface using different electrochemical methods. The structural conformation of DNA and hybridization between synthetic peptide nucleic acid (PNA) and DNA oligonucleotides were determined by the changes in the voltammetric peak of MB. The effect of probe, target, and ssDNA concentration upon the MB signal was investigated. Results demonstrated that MB could be used as an effective electroactive hybridization indicator for DNA biosensors.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA203%2F02%2F0422" target="_blank" >GA203/02/0422: New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

1

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

119-126

UT code for WoS article

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EID of the result in the Scopus database

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