Melting of cross-linked DNA V. Cross-linking effect caused by local stabilization of the double helix.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F03%3A17033293" target="_blank" >RIV/68081707:_____/03:17033293 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Melting of cross-linked DNA V. Cross-linking effect caused by local stabilization of the double helix.
Original language description
The stabilization of DNA can be affected by all the types of chemical modifications of DNA (interstrand cross-links, intrastrand cross-links or monofunctional adducts) which give rise to a strong enough local stabilization of the double helix. Our calculation demonstrates that an increase in stability by 25 to 30 kcal in the free energy of a single base pair of the double helix is sufficient for conserving the helicity of this base pair and preventing strand separation after melting of ordinary base pairs. For the situation where there is more then one stabilized site in a DNA duplex, a lower stabilization per site is sufficient (18 - 20 kcal). A substantial increase in DNA stability was found in various experimental studies for some metal-based antitumor compounds. Stretches of GC pairs more than 25 bp in length inserted into poly(AT) DNA also exhibit properties of stabilizing interstrand cross-links.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biomolecular Structure and Dynamics
ISSN
0739-1102
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
13
Pages from-to
533-545
UT code for WoS article
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EID of the result in the Scopus database
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