Magnetic bead-based hybridization assay for electrochemical detection of microRNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00427971" target="_blank" >RIV/68081707:_____/14:00427971 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2014.01.023" target="_blank" >http://dx.doi.org/10.1016/j.aca.2014.01.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2014.01.023" target="_blank" >10.1016/j.aca.2014.01.023</a>
Alternative languages
Result language
angličtina
Original language name
Magnetic bead-based hybridization assay for electrochemical detection of microRNA
Original language description
Aberrant expression of microRNAs (miRNAs), short non-coding RNA molecules regulating gene expression, is often found in tumor cells, making the miRNAs suitable candidates as cancer biomarkers. Electrochemistry is an interesting alternative to current standard methods of miRNA detection by offering cheaper instrumentation and faster assays times. In this paper, we labeled miRNA in a quick, simple, two-step procedure with electroactive complex of osmium(VI) and 2,2'-bipyridine, Os(VI) bipy, which specifically binds to the ribose at the 3'-end of the miRNA, and hybridized such labeled miRNA with biotinylated capture probe attached to the streptavidin magnetic beads. Labeled miRNA was then detected at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of the peak from the Os(VI) bipy label.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
813
Issue of the periodical within the volume
FEB2014
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
35-40
UT code for WoS article
000331556000004
EID of the result in the Scopus database
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