Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F13%3A00422262" target="_blank" >RIV/68081707:_____/13:00422262 - isvavai.cz</a>
Alternative codes found
RIV/00209805:_____/13:#0000415
Result on the web
<a href="http://dx.doi.org/10.1016/j.elecom.2013.04.009" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2013.04.009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.elecom.2013.04.009" target="_blank" >10.1016/j.elecom.2013.04.009</a>
Alternative languages
Result language
angličtina
Original language name
Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers
Original language description
Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and2,2'-bipyridine, Os(VI)bipy, specifically binding to the 3'-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labeling step with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture of other noncomplementary miRNAs was possible. (c) 2013 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochemistry Communications
ISSN
1388-2481
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
AUG2013
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
55-58
UT code for WoS article
000323024600014
EID of the result in the Scopus database
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