Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00431786" target="_blank" >RIV/68081707:_____/14:00431786 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/14:00435478
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2014.03.029" target="_blank" >http://dx.doi.org/10.1016/j.aca.2014.03.029</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2014.03.029" target="_blank" >10.1016/j.aca.2014.03.029</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface
Original language description
Electrochemical biosensors have the unique ability to convert biological events directly into electrical signals suitable for parallel analysis. Here we utilize specific properties of constant current chronopotentiometric stripping (CPS) in the analysisof protein and DNA-protein complex nanolayers. Rapid potential changes at high negative current intensities (I-str) in CPS are utilized in the analysis of DNA-protein interactions at thiol-modified mercury electrodes. P53 core domain (p53CD) sequence-specific binding to DNA results in a striking decrease in the electrocatalytic signal of free p53. This decrease is related to changes in the accessibility of the electroactive amino acid residues in the p53CD-DNA complex. By adjusting I-str and temperature, weaker non-specific binding can be eliminated or distinguished from the sequence-specific binding. The method also reflects differences in the stabilities of different sequence-specific complexes, including those containing spacers betw
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
828
Issue of the periodical within the volume
MAY2014
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
1-8
UT code for WoS article
000336336900001
EID of the result in the Scopus database
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