Combining Rational and Random Strategies in beta-Glucosidase Zm-p60.1 Protein Library Construction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00435973" target="_blank" >RIV/68081707:_____/14:00435973 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0108292" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0108292</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0108292" target="_blank" >10.1371/journal.pone.0108292</a>

Result language

angličtina

Original language name

Combining Rational and Random Strategies in beta-Glucosidase Zm-p60.1 Protein Library Construction

Original language description

Saturation mutagenesis is a cornerstone technique in protein engineering because of its utility (in conjunction with appropriate analytical techniques) for assessing effects of varying residues at selected positions on proteins' structures and functions.Site-directed mutagenesis with degenerate primers is the simplest and most rapid saturation mutagenesis technique. Thus, it is highly appropriate for assessing whether or not variation at certain sites is permissible, but not necessarily the most time-and cost-effective technique for detailed assessment of variations' effects. Thus, in the presented study we applied the technique to randomize position W373 in beta-glucosidase Zm-p60.1, which is highly conserved among beta-glucosidases. Unexpectedly, beta-glucosidase activity screening of the generated variants showed that most variants were active, although they generally had significantly lower activity than the wild type enzyme.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

SEP2014

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

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UT code for WoS article

000342685600060

EID of the result in the Scopus database

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