Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00447649" target="_blank" >RIV/68081707:_____/15:00447649 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/chem.201501307" target="_blank" >http://dx.doi.org/10.1002/chem.201501307</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201501307" target="_blank" >10.1002/chem.201501307</a>

Result language

angličtina

Original language name

Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA-Related Enzymatic Activities

Original language description

The dinuclear iron(II) supramolecular helicates [Fe2L3]Cl-4 (L= C25H20N4) bind to DNA through noncovalent (i. e., hydrogen-bonding, electrostatic) interactions and exhibit antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA-condensing agents. Notably, molecules of DNA in the presence of the M enantiomer of [Fe2L]Cl-4 do not form intermolecular aggregates typically formed by other condensing agents, such asspermidine or spermine. The helicates inhibit the activity of several DNA-processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site-specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe2L3]Cl-4 helicates on DNA-related enzymatic activities have been proposed.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA13-08273S" target="_blank" >GA13-08273S: Biophysical analysis of DNA condensation. Effect of new organometallic compounds of biological significance.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

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Volume of the periodical

21

Issue of the periodical within the volume

31

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

11189-11195

UT code for WoS article

000358216200034

EID of the result in the Scopus database

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