Fast-scan cyclic voltammetry with thiol-modified mercury electrodes distinguishes native from denatured BSA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00472036" target="_blank" >RIV/68081707:_____/15:00472036 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.elecom.2015.10.017" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2015.10.017</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.elecom.2015.10.017" target="_blank" >10.1016/j.elecom.2015.10.017</a>
Alternative languages
Result language
angličtina
Original language name
Fast-scan cyclic voltammetry with thiol-modified mercury electrodes distinguishes native from denatured BSA
Original language description
We studied native and denatured bovine serum albumin (BSA) at bare and dithiothreithol (DTT)-modified hanging mercury drop electrode (HMDE) by fast-scan cyclic voltammetry (fsCV) and chronopotentiometric stripping (CPS) to compare these methods for their ability to recognize changes in BSA structure. Using fsCV and bare HMDE, denatured BSA could be distinguished from its native form only between 10 and 20 V/s but at lower resolution than with CPS. At DTT-HMDE denatured BSA was recognized from native BSA in a wider range of scan rates suggesting new possibilities in development of voltammetric protein structure-sensitive sensing. (C) 2015 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA15-15479S" target="_blank" >GA15-15479S: New tools for research and diagnostics of diseases. Microfluidic reactors and electrochemistry for analysis of proteins and their glycosylation.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochemistry Communications
ISSN
1388-2481
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
DEC2015
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
3
Pages from-to
114-116
UT code for WoS article
000367124900027
EID of the result in the Scopus database
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