Modification of a Mercury Electrode with Different Thioalkanes: Structure-Sensitive Bovine Serum Albumin Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00491232" target="_blank" >RIV/68081707:_____/18:00491232 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/celc.201800275" target="_blank" >http://dx.doi.org/10.1002/celc.201800275</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/celc.201800275" target="_blank" >10.1002/celc.201800275</a>

Result language
angličtina
Original language name
Modification of a Mercury Electrode with Different Thioalkanes: Structure-Sensitive Bovine Serum Albumin Analysis
Original language description
Chronopotentiometric stripping (CPS) in combination with mercury electrodes is convenient for structure-sensitive protein analysis. By using this method, we tested the discrimination between native and denatured bovine serum albumin (BSA) at various alkanethiol self-assembled monolayers (SAMs). Our results showed that alkanethiol SAMs with different headgroups or different chain lengths had different influences on the efficiency of CPS in protecting BSA from the electric field-induced denaturation. SAMs with longer alkanethiol chain lengths were more efficient in protecting BSA from denaturation at negatively charged electrode surface than those formed by short-chain alkanethiols. Short alkanethiol SAMs could be prepared by co-adsorption with the protein on the electrode surface, which was not possible with the long-chain alkanethiols. CPS data were supported by impedance analysis. We believe that our work will be useful in the electrochemical sensing of nucleic acid-protein and protein-protein binding.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/GA18-18154S" target="_blank" >GA18-18154S: New tools of label-free electrochemical analysis of protein interactions with nucleic acids and proteins</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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