Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00472038" target="_blank" >RIV/68081707:_____/15:00472038 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/NPLANTS.2015.145" target="_blank" >http://dx.doi.org/10.1038/NPLANTS.2015.145</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/NPLANTS.2015.145" target="_blank" >10.1038/NPLANTS.2015.145</a>

Result language

angličtina

Original language name

Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system

Original language description

To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Plants

ISSN

2055-026X

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000364414200002

EID of the result in the Scopus database

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