Label-free electrochemical detection of singlet oxygen protein damage

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00456295" target="_blank" >RIV/68081707:_____/16:00456295 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.electacta.2015.11.104" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.11.104</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2015.11.104" target="_blank" >10.1016/j.electacta.2015.11.104</a>

Result language
angličtina
Original language name
Label-free electrochemical detection of singlet oxygen protein damage
Original language description
Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (O-1(2))-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2'-bipyridine)ruthenium(II) cation [Ru(bpy)(3)](2+) was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/GA13-00956S" target="_blank" >GA13-00956S: Emerging roles for the proteins of Anterior Gradient family in cancer development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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