Structure of a Stable G-Hairpin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00476559" target="_blank" >RIV/68081707:_____/17:00476559 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/17:00094788
Result on the web
<a href="http://dx.doi.org/10.1021/jacs.6b10786" target="_blank" >http://dx.doi.org/10.1021/jacs.6b10786</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/jacs.6b10786" target="_blank" >10.1021/jacs.6b10786</a>

Result language
angličtina
Original language name
Structure of a Stable G-Hairpin
Original language description
In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/ antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between NIcarbonyl symmetric and N1-carbonyl, N7-aminobase-pairingarrangements. Although the studied sequence first appears to possess a low capacity for base pairing, it forms a thermodynamically stable structure with a rather complex topology that includes a chain reversal arrangement of the backbone in the center of the continuous G-tract and 3' to5' stacking of the terminal residues. The structure reveals previously unknown principles of the folding of G rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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