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PHYSICOCHEMICAL METHODS AS A SUITABLE INSTRUMENT FOR SHORT DNA FRAGMENTS STUDY

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00075780" target="_blank" >RIV/00216224:14310/14:00075780 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    PHYSICOCHEMICAL METHODS AS A SUITABLE INSTRUMENT FOR SHORT DNA FRAGMENTS STUDY

  • Original language description

    Oligodeoxynucleotides (ODNs) are observed in the promoter region of the oncogens and of human telomeric DNA as a multiple repetitive C- or G- sequences. These sequences form tetraplex structures. C- rich ODNs form the so called i-motif, a hemiprotonatedC-C+ base pairing structures, at slightly acidic pH. G- rich ODNs form a G-quartet, made from four guanines and stabilized by a metal kation and can be folded into a G-tetraplex. We used electrochemical methods to study the behaviour of these compounds in buffered solutions with different pH, so we can comparatively evaluate the structure and fiction of each homo-ODN for better understanding their behaviour in solutions and on the charged interface. The study was completed by circular dichroism spektrato confirm the structure. Results should provide better understanding of these compounds, so the suitable biosensor could be designed.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Sborník příspěvků XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků

  • ISBN

    9788021068421

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    40-43

  • Publisher name

    Masarykova univerzita

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Jan 1, 2014

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article