PCNA is recruited to irradiated chromatin in late S-phase and is most pronounced in G2 phase of the cell cycle

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00485554" target="_blank" >RIV/68081707:_____/17:00485554 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/17:10364608

Result on the web

<a href="http://dx.doi.org/10.1007/s00709-017-1076-1" target="_blank" >http://dx.doi.org/10.1007/s00709-017-1076-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00709-017-1076-1" target="_blank" >10.1007/s00709-017-1076-1</a>

Result language

angličtina

Original language name

PCNA is recruited to irradiated chromatin in late S-phase and is most pronounced in G2 phase of the cell cycle

Original language description

DNA repair is a complex process that prevents genomic instability. Many proteins play fundamental roles in regulating the optimal repair of DNA lesions. Proliferating cell nuclear antigen (PCNA) is a key factor that initiates recombination-associated DNA synthesis after injury. Here, in very early S-phase, we show that the fluorescence intensity of mCherry-tagged PCNA after local micro-irradiation was less than the fluorescence intensity of non-irradiated mCherry-PCNA-positive replication foci. However, PCNA protein accumulated at locally irradiated chromatin in very late S-phase of the cell cycle, and this effect was more pronounced in the following G2 phase. In comparison to the dispersed form of PCNA, a reduced mobile fraction appeared in PCNA-positive replication foci during S-phase, and we observed similar recovery time after photobleaching at locally induced DNA lesions. This diffusion of mCherry-PCNA in micro-irradiated regions was not affected by cell cycle phases. We also studied the link between function of PCNA and A-type lamins in late S-phase. We found that the accumulation of PCNA at micro-irradiated chromatin is identical in wild-type and A-type lamin-deficient cells. Only micro-irradiation of the nuclear interior, and thus the irradiation of internal A-type lamins, caused the fluorescence intensity of mCherry-tagged PCNA to increase. In summary, we showed that PCNA begins to play a role in DNA repair in late S-phase and that PCNA function in repair is maintained during the G2 phase of the cell cycle. However, PCNA mobility is reduced after local micro-irradiation regardless of the cell cycle phase.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protoplasma

ISSN

0033-183X

e-ISSN

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Volume of the periodical

254

Issue of the periodical within the volume

5

Country of publishing house

AT - AUSTRIA

Number of pages

9

Pages from-to

2035-2043

UT code for WoS article

000411515200021

EID of the result in the Scopus database

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