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Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00488384" target="_blank" >RIV/68081707:_____/18:00488384 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14740/18:00100847 RIV/60076658:12310/18:43897417

  • Result on the web

    <a href="http://dx.doi.org/10.1002/anie.201712284" target="_blank" >http://dx.doi.org/10.1002/anie.201712284</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/anie.201712284" target="_blank" >10.1002/anie.201712284</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

  • Original language description

    C-rich DNA has the capacity to form a tetra-stranded structure known as an i-motif. The i-motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existence of these structures invivo has been missing. Whether i-motif structures form in complex environment of living cells is not currently known. Herein, using state-of-the-art in-cell NMR spectroscopy, we evaluate the stabilities of i-motif structures in the complex cellular environment. We show that i-motifs formed from naturally occurring C-rich sequences in the human genome are stable and persist in the nuclei of living human cells. Our data show that i-motif stabilities invivo are generally distinct from those invitro. Our results are the first to interlink the stability of DNA i-motifs invitro with their stability invivo and provide essential information for the design and development of i-motif-based DNA biosensors for intracellular applications.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Angewandte Chemie - International Edition

  • ISSN

    1433-7851

  • e-ISSN

  • Volume of the periodical

    57

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    5

  • Pages from-to

    2165-2169

  • UT code for WoS article

    000424879400024

  • EID of the result in the Scopus database