In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00585257" target="_blank" >RIV/68081707:_____/24:00585257 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/24:00136213
Result on the web
<a href="https://www.nature.com/articles/s41467-024-46221-y" target="_blank" >https://www.nature.com/articles/s41467-024-46221-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-024-46221-y" target="_blank" >10.1038/s41467-024-46221-y</a>
Alternative languages
Result language
angličtina
Original language name
In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells
Original language description
I-Motifs (iM) are non-canonical DNA structures potentially forming in the accessible, single-stranded, cytosine-rich genomic regions with regulatory roles. Chromatin, protein interactions, and intracellular properties seem to govern iM formation at sites with i-motif formation propensity (iMFPS) in human cells, yet their specific contributions remain unclear. Using in-cell NMR with oligonucleotide iMFPS models, we monitor iM-associated structural equilibria in asynchronous and cell cycle-synchronized HeLa cells at 37 degrees C. Our findings show that iMFPS displaying pH(T) < 7 under reference in vitro conditions occur predominantly in unfolded states in cells, while those with pH(T) > 7 appear as a mix of folded and unfolded states depending on the cell cycle phase. Comparing these results with previous data obtained using an iM-specific antibody (iMab) reveals that cell cycle-dependent iM formation has a dual origin, and iM formation concerns only a tiny fraction (possibly 1%) of genomic sites with iM formation propensity. We propose a comprehensive model aligning observations from iMab and in-cell NMR and enabling the identification of iMFPS capable of adopting iM structures under physiological conditions in living human cells. Our results suggest that many iMFPS may have biological roles linked to their unfolded states.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
2041-1723
Volume of the periodical
15
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1992
UT code for WoS article
001180394600033
EID of the result in the Scopus database
2-s2.0-85186873110