i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00488376" target="_blank" >RIV/68081707:_____/18:00488376 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gky035" target="_blank" >http://dx.doi.org/10.1093/nar/gky035</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gky035" target="_blank" >10.1093/nar/gky035</a>

Result language

angličtina

Original language name

i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

Original language description

i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examined the effect of four naturally occurring DNA base lesions on the folding and stability of the iM formed by the human telomere DNA sequence (C3TAA) 3C3T. The results demonstrate that the TAA loop lesions, the apurinic site and 8-oxoadenine substituting for adenine, and the 5-hydroxymethyluracil substituting for thymine only marginally disturb the formation of iM. The presence of uracil, which is formed by enzymatic or spontaneous deamination of cytosine, shifts iM formation towards substantially more acidic pH values and simultaneously distinctly reduces iM stability. This effect depends on the position of the damage sites in the sequence. The results have enabled us to formulate additional rules for iM formation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

1362-4962

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

1624-1634

UT code for WoS article

000426293300012

EID of the result in the Scopus database

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