Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00488411" target="_blank" >RIV/68081707:_____/18:00488411 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/18:00067624 RIV/00216224:14310/18:00102435

Result on the web

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2017.08.131" target="_blank" >10.1016/j.ijbiomac.2017.08.131</a>

Result language

angličtina

Original language name

Absence of differences among low, middle, and high molecular weight hyaluronan in activating murine immune cells in vitro

Original language description

Hyaluronan (HA) effects on immune response are suggested to be dependent on HA molecular weight (MW), as low MW HA should activate immune cells in contrast to high MW HA. However, some current studies do not support this conception and emphasize the importance of the form of preparation of HA, particularly with respect to its purity and origin. We compared the activation of mouse immune cells by HA samples (100 kDa, 500 kDa, and 997 kDa) prepared from HA originating from rooster comb, and HA samples (71 kDa, 500 kDa, and 1000 kDa) prepared from pharmacological grade HA originating from Streptococcus equi. Interestingly, in contrast to established theory, only middle and high MW HA originating from rooster comb induced the production of tumor necrosis factor-alpha by macrophages and in whole blood. Further, all tested preparations of HA failed to induce the expression of inducible nitric oxide synthase, the production of nitric oxide, or the expression of cyclooxygenase 2 in macrophages and splenocytes. Importantly, all HA samples originating from rooster comb were found to be contaminated by endotoxin (up to 1.23 EU/ml). Hence, low MW HA did not reveal itself to have significantly higher immunostimulatory activity compared to HA of higher MW. (C) 2017 Elsevier B.V. All rights reserved.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/LQ1605" target="_blank" >LQ1605: Translational Medicine</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

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Volume of the periodical

107

Issue of the periodical within the volume

FEB2018

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1-8

UT code for WoS article

000423892200001

EID of the result in the Scopus database

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