RNA synthesis is modulated by G-quadruplex formation in Hepatitis C virus negative RNA strand

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00491129" target="_blank" >RIV/68081707:_____/18:00491129 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41598-018-26582-3" target="_blank" >http://dx.doi.org/10.1038/s41598-018-26582-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-26582-3" target="_blank" >10.1038/s41598-018-26582-3</a>

Result language
angličtina
Original language name
RNA synthesis is modulated by G-quadruplex formation in Hepatitis C virus negative RNA strand
Original language description
DNA and RNA guanine-rich oligonucleotides can form non-canonical structures called G-quadruplexes or G4 that are based on the stacking of G-quartets. The role of DNA and RNA G4 is documented in eukaryotic cells and in pathogens such as viruses. Yet, G4 have been identified only in a few RNA viruses, including the Flaviviridae family. In this study, we analysed the last 157 nucleotides at the 3' end of the HCV (-) strand. This sequence is known to be the minimal sequence required for an efficient RNA replication. Using bioinformatics and biophysics, we identified a highly conserved G4-prone sequence located in the stem-loop IIy' of the negative strand. We also showed that the formation of this G-quadruplex inhibits the in vitro RNA synthesis by the RdRp. Furthermore, Phen-DC3, a specific G-quadruplex binder, is able to inhibit HCV viral replication in cells in conditions where no cytotoxicity was measured. Considering that this domain of the negative RNA strand is well conserved among HCV genotypes, G4 ligands could be of interest for new antiviral therapies.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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