Fishing for G-Quadruplexes in Solution with a Perylene Diimide Derivative Labeled with Biotins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00495172" target="_blank" >RIV/68081707:_____/18:00495172 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/chem.201802365" target="_blank" >http://dx.doi.org/10.1002/chem.201802365</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201802365" target="_blank" >10.1002/chem.201802365</a>

Result language

angličtina

Original language name

Fishing for G-Quadruplexes in Solution with a Perylene Diimide Derivative Labeled with Biotins

Original language description

A new fluorescent, non-cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G-quadruplexes with scarce or no affinity towards single- or double-stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G-quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80% of G-quadruplex structures from solution, even in the presence of an excess of single-stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2- over 3-tetrad quadruplexes, which constitutes a novel type of behavior.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

44

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

11292-11296

UT code for WoS article

000440908500009

EID of the result in the Scopus database

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