Anterior gradient-3 protein-antibody interaction at charged interfaces. Label-free chronopotentiometric sensing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00502570" target="_blank" >RIV/68081707:_____/19:00502570 - isvavai.cz</a>
Alternative codes found
RIV/00209805:_____/19:00078126
Result on the web
<a href="http://dx.doi.org/10.1016/j.electacta.2018.12.049" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2018.12.049</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2018.12.049" target="_blank" >10.1016/j.electacta.2018.12.049</a>
Alternative languages
Result language
angličtina
Original language name
Anterior gradient-3 protein-antibody interaction at charged interfaces. Label-free chronopotentiometric sensing
Original language description
We developed a fast, simple, label-free method useful for the study of interactions between an antibody (Ab) and antigen based on chronopotentiometric stripping analysis and the catalytic hydrogen evolution reaction. The specific interaction of the Ab, adsorbed at a mercury electrode, with AGR3 protein induced a significant increase in chronopotentiometric peak H in comparison to both the CPS response of the Ab alone and that after incubation with nonspecifically binding proteins. Qualitatively similar results were obtained with another polyclonal antibody specific for AGR2 protein. The demonstrated results, along with previous findings indicate that the proposed technique shows promise as a new option for studying the dynamics, not only of surface-attached antibody-antigen complexes, but also other protein-protein interactions. (C) 2018 Elsevier Ltd. All rights reserved.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica acta
ISSN
0013-4686
e-ISSN
—
Volume of the periodical
297
Issue of the periodical within the volume
FEB 20 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
974-979
UT code for WoS article
000455642500107
EID of the result in the Scopus database
—