Does Raman spectroscopy recognize different G-quadruplex arrangements?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00520332" target="_blank" >RIV/68081707:_____/19:00520332 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/20:10406088
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5772" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5772</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jrs.5772" target="_blank" >10.1002/jrs.5772</a>
Alternative languages
Result language
angličtina
Original language name
Does Raman spectroscopy recognize different G-quadruplex arrangements?
Original language description
In this work, we demonstrate the underused potential of Raman spectroscopy in detecting formation of DNA G-quadruplex (G4) and differentiating between various G4-folding topologies. As model structures, we used a 22-mer human telomere fragment AG(3)(TTAG(3))(3) (denoted here as wt) and its two variants with adenine abasic lesions at the position 19 (ap19) or at the positions 7, 13, and 19 (ap7-13-19), the structures of which were previously characterized. These oligonucleotides adopt three basic G4-folds: a basket-type form for wt in Na+, a (3+1) hybrid form for ap19 in K+, and a parallel G4 for ap7-13-19 in K+, the last one furthermore stabilized at increased temperatures. We show that although wt and ap7-13-19 in Na+ adopt predominately antiparallel G4-folds, these oligonucleotides in K+ display several Raman features of parallel G4 folds. Interestingly, ap19 has a Raman spectrum with some features of parallel G4 folds in both Na+ and K+ solutions. Raman signals indicative of parallel G4 are most obvious with ap7-13-19 in K+. The population of ap7-13-19 guanosines (unlike of wt and ap19 in K+) in the C2'-endo/anti geometry gradually increases with increasing temperature, reaching its maximum at similar to 50 degrees C, where an all-parallel topology is observed. In summary, Raman spectroscopy has been proven to distinguish between different conformational properties of G4-DNA structures, with reliability comparable with circular dichroism spectra, and with the potential to provide additional structural information.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Raman Spectroscopy
ISSN
0377-0486
e-ISSN
1097-4555
Volume of the periodical
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Issue of the periodical within the volume
NOV 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
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UT code for WoS article
000494269300001
EID of the result in the Scopus database
2-s2.0-85074794217