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Does Raman spectroscopy recognize different G-quadruplex arrangements?

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00520332" target="_blank" >RIV/68081707:_____/19:00520332 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11320/20:10406088

  • Result on the web

    <a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5772" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5772</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/jrs.5772" target="_blank" >10.1002/jrs.5772</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Does Raman spectroscopy recognize different G-quadruplex arrangements?

  • Original language description

    In this work, we demonstrate the underused potential of Raman spectroscopy in detecting formation of DNA G-quadruplex (G4) and differentiating between various G4-folding topologies. As model structures, we used a 22-mer human telomere fragment AG(3)(TTAG(3))(3) (denoted here as wt) and its two variants with adenine abasic lesions at the position 19 (ap19) or at the positions 7, 13, and 19 (ap7-13-19), the structures of which were previously characterized. These oligonucleotides adopt three basic G4-folds: a basket-type form for wt in Na+, a (3+1) hybrid form for ap19 in K+, and a parallel G4 for ap7-13-19 in K+, the last one furthermore stabilized at increased temperatures. We show that although wt and ap7-13-19 in Na+ adopt predominately antiparallel G4-folds, these oligonucleotides in K+ display several Raman features of parallel G4 folds. Interestingly, ap19 has a Raman spectrum with some features of parallel G4 folds in both Na+ and K+ solutions. Raman signals indicative of parallel G4 are most obvious with ap7-13-19 in K+. The population of ap7-13-19 guanosines (unlike of wt and ap19 in K+) in the C2'-endo/anti geometry gradually increases with increasing temperature, reaching its maximum at similar to 50 degrees C, where an all-parallel topology is observed. In summary, Raman spectroscopy has been proven to distinguish between different conformational properties of G4-DNA structures, with reliability comparable with circular dichroism spectra, and with the potential to provide additional structural information.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10403 - Physical chemistry

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Raman Spectroscopy

  • ISSN

    0377-0486

  • e-ISSN

    1097-4555

  • Volume of the periodical

  • Issue of the periodical within the volume

    NOV 2019

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    12

  • Pages from-to

  • UT code for WoS article

    000494269300001

  • EID of the result in the Scopus database

    2-s2.0-85074794217